IMMUNE CELLS IN THE ENDOMETRIUM DURING MID-LUTEAL PHASE

To evaluate the associations among immune cells (T-cells, B-cells, Macrophages and NK cells) in human endometrium during mid-luteal phase of menstrual cycle. Endometrial biopsies were obtained from 116 women (7 days after LH surge) natural cycles. The presence CD3-positive (T-cells), CD4-positive (T-helpers), CD8-positive (T-killers), CD14-positive (Macrophages), CD68-positive CD56-positive (NK CD79α-positive (B-cells) endometrial samples was confirmed by immunohistochemical staining using CD3 (BRB063, Zytomed Systems), CD4 (IS649, Dako), CD8 (1-CD040-02, Quartett), CD14 (Е-AB-71017, Elabscience), CD68 (IS613, CD56 (А00121-0007, ScyTek), CD79α (IS621, Dako) antibodies, respectively. percentage positively stained stromal evaluated Image-J software (NIH, Maryland, USA) multiple sections. Statistical analysis performed Pearson’s correlation test SPSS v.21. mean cells), 1.13±0.60%, 0.37±0.09%, 0.74±0.39%, 1.31±0.57%, 1.02±0.02%, 0.88±0.84% 0.19±0.06%, A significant positive observed between each pair analysed (p<0.001). strongest relation macrophages T killer (R=0.961) (R=0.964). In conclusion we observe CD3-positive, Furthermore correlations present found.